The Spot Count module in Imaris ® Suite from Bitplane AG enabled quantification of confocal images and produced spreadsheets of the cell counts. It was necessary to compare these transgenics with one another as well as with controls. One study involved quantifying approximately 100 cells in five transgenic mice representing three different disease models. I have been able to analyze an extremely large data set within a matter of minutes.
The possibilities are endless in how GraphPad Prism can perform biostatistics. Prior knowledge of statistics is not necessary, making it easier for one to concentrate on the data rather than trying to learn how to use the software. The best aspect of this program is that it provides step-by-step instructions on organizing, analyzing, and graphing data sets. It incorporates biostatistics and scientific graphing in an all-inclusive program enabling one to pick the appropriate analytical tests and produce a variety of graphs. Prism from GraphPad has made it extremely easy to analyze data and graph experiments in a very professional and scientific manner. It is also important to be able to graphically represent the data in order to portray the scientific significance of an experiment. However, it can be overwhelming to organize and interpret spreadsheets without having a good understanding of biostatistics and analysis software.
These programs can quantify various biological processes including protein colocalization, fluorescence intensity, and structural volume. There are several programs on the market, such as Imaris ® Suite from Bitplane AG, that allow one to generate data from images. Digital quantification has made it easier to increase experimental throughput and thus, produce large data sets.
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